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KMID : 0380020070220010053
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Korean Journal of Biotechnology and Bioengineering
2007 Volume.22 No. 1 p.53 ~ p.57
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Biochemical Characterization of Lectin Purified from Kidney Bean Seedling
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Roh Kwang-Soo
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Abstract
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We have studied biochemical characterization of lectin purified from kidney bean seedling through PBS extraction, precipitation, and Sephadex G-100 affinity chromatography. The lectin was agglutinated by rabbit erythrocytes. This lectin analyzed by SDS-PAGE is a tetramer composed of two subunits with molecular weights of 46 and 44 kDa. The optimal temperature and thermal stability of the lectin was 30 and 40-80, respectively. The maximal pH of this lectin was pH 8.2.
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KEYWORD
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Hemagglutination activity, kidney bean seedling, lectin
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